早期，鲑鱼和(he)鱿(you)鱼是美国及海外地区主要的(de)(de)(de)(de)(de)渔业(ye)资源，人(ren)们为了提高鱼类的(de)(de)(de)(de)(de)货架(jia)期，开(kai)始(shi)(shi)设(she)想臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)冰(bing)(bing)(bing)(bing)对新鲜鱼的(de)(de)(de)(de)(de)保(bao)鲜，初(chu)期试验(yan)是采用(yong)(yong)塑(su)料瓶盛装臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)水(shui)(shui)置于-80℃ 下(xia)冷(leng)冻(dong)成(cheng)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)冰(bing)(bing)(bing)(bing)，其在(zai)(zai)热碘化(hua)钾溶(rong)液中(zhong)(zhong)融化(hua)散发出臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)特殊气味，如此(ci)发现臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)在(zai)(zai)冰(bing)(bing)(bing)(bing)中(zhong)(zhong)能够稳(wen)定(ding)存在(zai)(zai)，并随着(zhe)冰(bing)(bing)(bing)(bing)块融化(hua)缓慢释放。当水(shui)(shui)的(de)(de)(de)(de)(de)pH=4.0，水(shui)(shui)温接(jie)近0℃，臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)气体流(liu)量2.5L/min，混合泵出水(shui)(shui)压力(li)0.2MPa的(de)(de)(de)(de)(de)条件(jian)下(xia)进行(xing)循环混合，能制(zhi)出含(han)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)115.3mg/kg的(de)(de)(de)(de)(de)高浓(nong)(nong)(nong)度(du)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)水(shui)(shui)，然后用(yong)(yong)快(kuai)速(su)(su)制(zhi)冰(bing)(bing)(bing)(bing)机可(ke)制(zhi)出含(han)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量高达(da)16.7 mg/kg的(de)(de)(de)(de)(de)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)冰(bing)(bing)(bing)(bing)。臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)冰(bing)(bing)(bing)(bing)在(zai)(zai)-18 ℃ 的(de)(de)(de)(de)(de)贮(zhu)藏过程(cheng)中(zhong)(zhong)，初(chu)始(shi)(shi)时冰(bing)(bing)(bing)(bing)中(zhong)(zhong)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)浓(nong)(nong)(nong)度(du)会有明显的(de)(de)(de)(de)(de)降低(di)(di)，而贮(zhu)藏5～30d时，冰(bing)(bing)(bing)(bing)的(de)(de)(de)(de)(de)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)浓(nong)(nong)(nong)度(du)基本能维持稳(wen)定(ding)不变。水(shui)(shui)温越低(di)(di)，臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)水(shui)(shui)冻(dong)结速(su)(su)率(lv)越快(kuai)，臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)损失越小，用(yong)(yong)液氮(-196℃)制(zhi)备的(de)(de)(de)(de)(de)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)冰(bing)(bing)(bing)(bing)浓(nong)(nong)(nong)度(du)很高；并且臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)含(han)量越高的(de)(de)(de)(de)(de)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)冰(bing)(bing)(bing)(bing)，常温下(xia)释放的(de)(de)(de)(de)(de)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)浓(nong)(nong)(nong)度(du)也(ye)越高。在(zai)(zai)装有超纯水(shui)(shui)的(de)(de)(de)(de)(de)5L密闭容器(qi)通入流(liu)量为0.5L/min臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)气体5min，可(ke)制(zhi)得臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)水(shui)(shui)浓(nong)(nong)(nong)度(du)为20.8mg/kg，用(yong)(yong)制(zhi)冰(bing)(bing)(bing)(bing)模(mo)(mo)具迅速(su)(su)置于-50℃低(di)(di)温速(su)(su)冻(dong)30s，制(zhi)得臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)冰(bing)(bing)(bing)(bing)中(zhong)(zhong)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)浓(nong)(nong)(nong)度(du)可(ke)达(da)18.0mg/kg，可(ke)用(yong)(yong)于治(zhi)疗口腔疾病(bing)、感(gan)染类皮(pi)肤(fu)病(bing)等。将(jiang)流(liu)速(su)(su)为2t/h 的(de)(de)(de)(de)(de)低(di)(di)温水(shui)(shui)(3℃)与流(liu)速(su)(su)为40g/h的(de)(de)(de)(de)(de)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)在(zai)(zai)0.45MPa 的(de)(de)(de)(de)(de)压力(li)下(xia)充分混合制(zhi)成(cheng)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)水(shui)(shui)，使用(yong)(yong)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)浓(nong)(nong)(nong)度(du)在(zai)(zai)线监(jian)测传感(gan)器(qi)调整臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)发生(sheng)器(qi)的(de)(de)(de)(de)(de)驱动电流(liu)和(he)驱动频率(lv)，使臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)水(shui)(shui)中(zhong)(zhong)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)浓(nong)(nong)(nong)度(du)保(bao)持在(zai)(zai)149～150mg/kg；预先制(zhi)成(cheng)的(de)(de)(de)(de)(de)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)水(shui)(shui)输入冷(leng)冻(dong)模(mo)(mo)具中(zhong)(zhong)，在(zai)(zai)-10℃温度(du)的(de)(de)(de)(de)(de)冷(leng)冻(dong)设(she)备中(zhong)(zhong)进行(xing)初(chu)步(bu)成(cheng)型，使其表面在(zai)(zai)120s内(nei)快(kuai)速(su)(su)形成(cheng)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)冰(bing)(bing)(bing)(bing)膜(mo)，膜(mo)的(de)(de)(de)(de)(de)体积占(zhan)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)水(shui)(shui)总(zong)体积的(de)(de)(de)(de)(de)3%，再将(jiang)初(chu)级臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)冰(bing)(bing)(bing)(bing)输送至另一冷(leng)冻(dong)设(she)备中(zhong)(zhong)，在(zai)(zai)低(di)(di)于-20℃下(xia)冷(leng)冻(dong)2h，然后在(zai)(zai)-25℃下(xia)冷(leng)冻(dong)1h进行(xing)包装，在(zai)(zai)-30℃下(xia)冷(leng)冻(dong)5h形成(cheng)成(cheng)品臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)冰(bing)(bing)(bing)(bing)，该臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)冰(bing)(bing)(bing)(bing)中(zhong)(zhong)臭(chou)(chou)(chou)(chou)(chou)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)浓(nong)(nong)(nong)度(du)为13～14mg/kg。

       目前，较(jiao)为成(cheng)熟的(de)臭(chou)氧(yang)(yang)(yang)冰(bing)(bing)制(zhi)备(bei)(bei)技(ji)术(shu)是(shi)用(yong)低(di)压电解水产生高(gao)浓度、高(gao)纯度臭(chou)氧(yang)(yang)(yang)气(qi)体(ti)，再将臭(chou)氧(yang)(yang)(yang)与水混合(he)成(cheng)臭(chou)氧(yang)(yang)(yang)水，利用(yong)低(di)温设备(bei)(bei)冷冻成(cheng)臭(chou)氧(yang)(yang)(yang)冰(bing)(bing)。这其(qi)中臭(chou)氧(yang)(yang)(yang)水冷冻成(cheng)臭(chou)氧(yang)(yang)(yang)冰(bing)(bing)的(de)方法(fa)比较(jiao)单一，一种(zhong)是(shi)将臭(chou)氧(yang)(yang)(yang)水连接(jie)快速制(zhi)冰(bing)(bing)机，直接(jie)产出臭(chou)氧(yang)(yang)(yang)冰(bing)(bing)，这种(zhong)快速制(zhi)冰(bing)(bing)机选材需(xu)要对臭(chou)氧(yang)(yang)(yang)具有良好的(de)抗性(如氟材料(liao)、SS304不锈钢、钛钢等)；另一种(zhong)是(shi)把预制(zhi)的(de)臭(chou)氧(yang)(yang)(yang)水装入(ru)容器内密封，然后(hou)置于低(di)温环境下速冻(如液(ye)氮、低(di)温乙醇、超低(di)温冰(bing)(bing)箱(xiang)或冷库等)。

       除(chu)了将(jiang)预先制(zhi)成的(de)(de)臭(chou)氧(yang)(yang)(yang)水利(li)用(yong)(yong)低(di)温设(she)备冻结成臭(chou)氧(yang)(yang)(yang)冰(bing)(bing)外(wai)，后制(zhi)作包含氧(yang)(yang)(yang)气(qi)气(qi)泡且密度为550～910 kg/m3 的(de)(de)冰(bing)(bing)，再利(li)用(yong)(yong)波长为180～242 nm的(de)(de)紫外(wai)线对所制(zhi)作的(de)(de)冰(bing)(bing)块照射，可以(yi)使冰(bing)(bing)中的(de)(de)氧(yang)(yang)(yang)气(qi)臭(chou)氧(yang)(yang)(yang)化制(zhi)成臭(chou)氧(yang)(yang)(yang)冰(bing)(bing)。除(chu)此之外(wai)，还可以(yi)考(kao)虑(lv)臭(chou)氧(yang)(yang)(yang)与其他(ta)技术结合(he)，拓展臭(chou)氧(yang)(yang)(yang)冰(bing)(bing)的(de)(de)应(ying)用(yong)(yong)范围(wei)。例如，流(liu)(liu)化冰(bing)(bing)具有快速降(jiang)温的(de)(de)效果，但使用(yong)(yong)期间易受(shou)到水产品表(biao)面(mian)微生物的(de)(de)交叉(cha)污染，而将(jiang)流(liu)(liu)化冰(bing)(bing)与臭(chou)氧(yang)(yang)(yang)结合(he)，可以(yi)充(chong)分利(li)用(yong)(yong)流(liu)(liu)化冰(bing)(bing)的(de)(de)降(jiang)温功能(neng)和(he)臭(chou)氧(yang)(yang)(yang)的(de)(de)杀菌效果。